Well-stacked Muslin Moth

UPDATE: 9th April 2020: First Muslin to the lure, conventionally photographed from three angles on stone.

The male Muslin Moth (Diaphora mendica [Clerck, 1759]) that I saw in the trap these last couple of mornings was there again today. I know, because he has a little snick out of the end of his left antenna. I was hoping a female might turn up, their wings are muslin-white, but the only other moth in the trap was a solitary Hebrew Character.

Muslin Moth

Anyway, the Muslin’s arrival gave me the opportunity to try out some more focus stacking. This time I used a couple of free tools. The first a controller for my Canon dSLR, digiCam Control. This software lets you control you dSLR via a USB cable from your computer and has builtin focus stacking (and many other functions).

I used its simple focus stacking to take a focus-bracketed set of four photos of the moth illuminated with an LED ring flash and natural light from my “studio” window (it’s just our back bedroom, which I use as an office). Anyway, each of the four photographs has its focus from near to far away from the camera. So the moth is pin sharp in each photo but only in a certain plane parallel to the camera’s sensor. The depth-of-field is very short with a macro lens at close quarters even with a small-ish aperture of f/9.5.

I then combined (automated process) the four shots using another piece of free software, CombineZP. I used what seemed to be the simplest option “Do Stack” and the resulting composite image was generated in a couple of minutes.

All very quick and easy. I am sure with practice and more attention to the details of optimising each piece of software and perhaps the lighting for the subject, I reckon it would be possible to get even better sharper shots, without having to spend hundreds of pounds on new hardware.

I also did a sequence of face-on portrait shots with the moth, automatically aligned them in CombineZP and then applied the “Do Stack” command, great result.

Focus stacking an Angle Shades moth

Yesterday, I had a Muslin moth to photograph. Today, I had another go at focus stacking a macro shot, with an Angle Shades (Phlogophora meticulosa).

Focus stacked moth, note how from nose to tail the image is largely in focus

Focus stacking involves taking essentially the same photo several times but focusing first on the foreground, then the mid, then the farthest point on the subject. You can take as many shots as you like to “bracket” the image and get a sequence of shots that have the whole of the object in focus at some point in each photo. As you can see in the photo above.

It works best if you set the camera up on a tripod and take the series of photos using magnified LiveView and manually focusing on different parts of the subject. There are automated systems (software and hardware) and some cameras have focus bracketing built in (think of it as the focusing analogue of exposure bracketing, which lets you create high dynamic range (HDR) photos.

Once you have your set of focus-bracketed photos, you can then use a photo editor to blend them into a single composite image where pretty much all of the shot is in focus. The technique overcomes the very shallow depth-of-field you have with a small aperture when shooting taking close-ups. That said, the technique works to extend DoF for any type of photo.

Today’s subject is the beautifully patterned Angle Shades moth (who said moths were dull and grey?). I had it sat in a pot and perched that on an old patterned chair. If I could have persuaded it out of the pot without it flying away, you could have seen better just how well camouflaged this moth is against such William Morris style arts and crafts prints. In the wild, of course, it finds itself beautifully camouflaged among multicoloured and dappled foliage.

Conventional, single shot from above.

Focus stacking a Muslin moth

It has been a bit quiet on the new-to-the-garden moths, basically because it’s still quite cold and the night-flyers aren’t out in great numbers yet. Nevertheless, a male Muslin moth (Diaphora mendica) turned up last night. Hashtag #floof. Here he is “focus stacked” using half a dozen macro close-ups and Zerene Stacker. The aerial view is a single shot.

Photo stacked Muslin moth

The females don’t have the big pheromone antennae and are white with the black spots.

Overhead view male Muslin moth

The other moths that are around and that have turned up in varying numbers in the last month or so include: Common Quaker, Small Quaker, Twin-spotted Quaker, Hebrew Character, Clouded Drab, Early Grey, Twenty Plume, Common Plume, Early Thorn, Garden Carpet, Nut-tree Tussock, Agonopterix yeatiana, March Moth, The Chestnut, Double-striped Pug, March Dagger, Dotted Border, Pale Pinion, Oak Beauty, Pale Brindled Beauty, Acleris cristana.

Photos of all these updated with the new entries can be found in my Mothematics gallery on Imaging Storm, also includes butterflies, but they’re really just a type of moth, anyway.

Moths, dull, grey, night-flying insects?

Ask anyone who isn’t a moth-er to describe a moth and usually terms such as dull, grey, brown, night-flying, drab, dingy, useless are the ones that arise. Someone might go so far as to describe them as the boring relatives of butterflies. Well semantics aside, butterflies are just a sub-group of the moths, they’re all Lepidoptera, but they’re anything but useless and many of them fly during the day and are incredibly vivid and bright.

Perhaps the most vivid and bright of the British species is a moth I’d not seen until today, only in books. It is the Emperor Moth, Saturnia pavonia, the only member of the Saturniidae, the silk moths, found in the British Isles.

The males are very brightly coloured, the females a version where the colours look as if they have been desaturated. The male flies during the day, the female at night. Both male and female have a vivid spot on each fore- and hind-wing that give them the appearance of having two pairs of eyes looking back at a predator. The species is actually fairly common across the British Isles although it favours heathery heathland and open country, but that does include Fenland, of which we have plenty hereabouts.

Female Emperors [should that be Empress moths? Ed.*] exude a pheromone to alert the day-flying males to their presence and their urge to mate. The males can detect picograms of sex pheromone on the wing with their feathery antennae. Purportedly, they can sniff out a female from up to ten miles.

Other Saturniidae moths in Japan and the Americas seem to use hexadecadienals and esters of those compounds as their sex pheromones. I’m yet to find a paper that isolated and characterised the sex pheromone of S pavonia. Nevertheless, you can buy a little lure impregnated with the sex pheromone. A moth-er might hang such a lure in the garden on a sunny and breezy day in the hope of attracting an Emperor, which is what I did.

First sighting was today. He wouldn’t settle  and I couldn’t safely net him, so I snapped away 100+ shots and maybe got 4 where the moth is in focus and in the frame.

*There is no “Ed.” it’s just me.

Luring in the Emperor

I ordered a pheromone lure for the Emperor Moth from ALS today. It’s just arrived and now it’s hanging in one of those little laundry tab string bags, out in the garden, blowing in the breeze purportedly drawing in the male Saturnia pavonia…but nothing yet. It’s getting late in the day for this day-flying moth, so I might pack the lure away in a freezer bag in the freezer and try again tomorrow. Intriguingly, the female which looks like a greyed out version of the male is a night-flyer.

Meanwhile, I cannot find the chemical identity of the female sex pheromone for this species. I can’t seem to find a reference in my usual journal searching, although three US Saturnia species apparently use a long-chain aldehyde, (E4, Z9)-tetradecadienal. I will keep searching. This is where my passion for biology and chemistry overlap, you might say.

UPDATE: I discovered that S pavonia had two older names, not just Pavonia pavonia, but Eudia pavonia. As soon as I learned the latter, it was easy to find a research paper discussing the pheromone – (Z)-6,(Z)-11-hexadecadien-1-yl acetate, which is a close relative of another sex attractant pheromone, gossyplure, which is used in commercial breeding control of pest moths. Read more about it here.

This species is the only member of the Saturniidae that lives in the British Isles, it is found almost everywhere here from heathland to fenland, so there is a chance of at least one turning up. It is the poster-boy of the moth world. You can go away and look it up if you like, but I’ll only post a photo when I’ve snapped one of my own.

Nut-tree Tussock (Colocasia coryli)

Pleased to see something more colourful and patterned than the Common Quakers in the actinic moth trap last night. A quick lookup in the book confirmed it as Nut-tree Tussock (Colocasia coryli). Very distinctive, but fairly common in Southern England.

The species likes Birch, Hawthorn, and Hornbeam, none of which are present in our garden, although there are birches fairly nearby. This specimen is a female? How do I know? 47 eggs laid in the pot by morning. I will leave them to hatch and release them on to a suitable deciduous tree once they do.

According to UKMoths, the species flies April to June and July to September in the south (double brooded); May to June in the north.

Later early moths

Update: Adding at least one new-for-me species every couple of days now to my mothematics gallery.  Most interesting and fascinating were the March Moth, the Twenty-plume, and the Nut-tree Tussock (which laid 47 eggs and I am going to attempt to get them to hatch).

Female Nut-tree Tussock (Colocasia coryli)

Previously, I posted about some of the early moths (early in the year, that is, as opposed to Early Moths, a species of that name) that have been attracted to the actinic light of the trap.

Diurnea fagella, the March Dagger Moth

On the evening of the 14th March 2019, it was relatively uncold, cloudy and pretty much windless.

Twin-spotted Quaker

There was a definite upturn in numbers and diversity to the trap: 4x Small Quaker, 10x Common Quaker, 3x Clouded Drab, 1xEarly Grey, and Double-striped Pug. That was the first Pug of the year, saw lots of that species and its relatives during the summer of 2018. Diurnea fagella was hanging on the LED fairylights on a cultivated honeysuckle in our garden).

Double-striped Pug

By morning, the Pug had gone but a Hebrew Character and a Twin-spotted Quaker were in residence in the trap (all ethically released after logging and photographing, of course.

Early Grey

Early moths

I started “lighting up” again for the 2019 season a couple of weeks ago. I don’t mean I’ve taken to smoking, I’m referring to moth-ing. I’m still using the borrowed and now bought homemade scientific moth trap. It is a 40-Watt actinic light Robinson style trap and was built by my cabinetmaker and amateur luthier friend Rob, whom I’ve mentioned previously (see Mothley Crew).

Common Quaker
Pale Brindled Beauty

Anyway, despite frosts, wind, and rain there have been a few early moths turning up in the trap over the last fortnight or so. I should note the 12-year old UV-A bulb was looking worn when illuminated so I have replaced it and am hoping for great things over the coming weeks and months.

Hebrew Character

First to appear were the Common Quakers (Orthosia cerasi) of which there have been several over the weeks. I should point out that the first moth I saw this year was 15th February in the front garden and was what made me think it might be worth lighting up again, Common Plume (Emmelina monodactyla). After the CQs, there was the relatively rare Acleris cristana on the trap, but not in it, Pale Brindled Beauty (Phigalia pilosaria), again on the trap in the evening and then in it by morning light. Hebrew Character (Orthosia gothica) moths started to show up by 23rd February and I’ve seen several not to be confused with their bristly relatives, which come later, Setaceous Hebrew Character (Xestia c-nigrum).

Oak Beauty
Dotted Border

Then, I had Oak Beauty (Biston strataria), which lives up to its name and gives me some hope that I might get other oak-loving species much later in the year, such as the elusive Mervs and Decembers. Next was Dotted Border (Agriopis marginaria) (also saw several of this species in the gents at NT Dunwich Heath), bizarrely, but perhaps not unsurprisingly.

Early Grey

Then Clouded Drab (Orthosia incerta), Small Quaker (Orthosia cruda), Twin-spotted Quaker (Anorthoa munda), and Early Grey (Xylocampa areola) on 8th March. Meanwhile, there was a Yellow Horned (Achlya flavicornis) at Dunwich, which I mentioned in my Dartford Warbler blog post about the site.

 

Yellow Horned

As new moths appear in the trap each evening, I’ll add them to my Mothematics gallery. I usually also post to the Facebook group UK Moths Flying Tonight.

Oak Beauty (Biston strataria)

I missed out on the Merveille du Jour moths late last year, had a few November moths, and a Winter moth, but no December moths. I was beginning to think that our garden environment was too far away from oak trees for some of the species that are attracted to oaks.

But, then having started “lighting up” again a few days ago been graced with the presence of numerous Common Quaker the larvae (caterpillars) of which prefer deciduous trees, including oak and willows. Then I had a couple of Pale Brindled Beauty (Phigalia pilosaria), which also favour oak. And, best of the bunch in terms of patterning and antennae, an Oak Beauty (Biston strataria). The Dotted Border is also a fan of a range of deciduous trees.

Oak Beauty
Oak Beauty
Oak Beauty
Oak Beauty showing its patterning and antennae
Dotted Border
Dotted Border almost camouflaged on a business card from a local pub

Sex antenna

TL:DR – The males of many species of moth have feathery antennae to detect the sex attractant pheromones released by the females to allow the males to locate a potential mate, often from several miles away.


That feathery protuberance on this moth (Pale Brindled Beauty, Phigalia pilosaria) is one of a pair of antennae. What you cannot see clearly in my photo is that it’s fractal with each tiny hair on the main stem having its own array of tiny hairs and so on down to the molecular level.

Feathery antennae like this are found only on male moths and are basically its sex radar. They can catch a few molecules of female moth sex attractant pheromone on the breeze sometimes coming from miles away and guiding the male to where the female might be found. The female of this particular species has no wings and so the male must go to her to mate.