Science – Page 13 – David Bradley

Nocturnal pollination #MothsMatter

TL:DR – Many species of moth are important nocturnal pollinators.

2018 was the year I took a serious interest in studying moths in my garden having been loaned a scientific mothtrap by a friend in the village. It was late July that year that I fell in love with the “nocturnal” Lepidoptera.

By the September, I’d recorded about 120 species of animal I’d never noticed, seen, nor observed ever before. In subsequent years I added yet more species. There are about 1600 moth species in the UK and I have ticked 464 (as of 1 April 2023). That same month there was some research demonstrating once again why moths matter and why we should not dismiss these remarkable creatures. The paper:

Construction, validation, and application of nocturnal pollen transport networks in an agro-ecosystem: a comparison using light microscopy and DNA metabarcoding

Callum J. Macgregor, James J. N. Kitson, Richard Fox, Christoph Hahn, David H. Lunt, Michael J. O. Pocock, Darren M. Evans First published: 17 September 2018 https://doi.org/10.1111/een.12674

The study compared two methods, light microscopy and DNA metabarcoding, for constructing pollen-transport networks of nocturnal moths, with a focus on their feeding mouthparts. The researchers found that DNA metabarcoding detected more pollen on individual moths and more interactions per moth species compared to microscopy.

The results also showed that the pollen-transport network metrics differed between the two methods due to their varying abilities to detect multiple pollen types per moth and to separate morphologically similar or related pollen. The team also demonstrated some unexpected moth-plant interactions using metabarcoding, revealing new insights into nocturnal pollination systems.

The authors suggest that while the two methods revealed similar yet distinct networks, the potential applications of metabarcoding for studying plant-pollinator interaction networks, especially for understudied pollinators like moths, are encouraging.

Trip to Teesdale

TL:DR – Brief spot of garden birding with friends in Teesdale and some sightseeing.

We spent a lovely couple of days with wonderful friends at their place in Teesdale…unfortunately I’ve been struggling to walk with an ankle injury so the usual long country walks and sightseeing were off the agenda, but we did get to toddle around the fabulous Bowes Museum in Barnard Castle and to sample some local ale at the local pub for local people (and their friends).

Through-window shot of male Great Spotted Woodpecker

Our friends had also arranged a fabulous selection of birds to use their garden bird feeders while we were there: Brambling, Nuthatch, Coal Tit, Great Spotted Woodpecker (M+F), Redpoll, Goldfinch, Long-tailed Tit, Great Tit, Blue Tit, Dunnock, Robin, Chaffinch, Jackdaw, Rook (either or both of which repeatedly knocked the feeders on to the lawn), Blackbird, House Sparrow, Starling (mainly in the trees not on the feeders); also Chiff Chaff calling from one of their trees. Didn’t see Tawny Owl on this visit.

Typical Teesdale — Teesdale village view

The Tees at Barnard Castle was too rough and high within my walking range to catch sight of Dippers or wagtails fishing this time, but there were Mallard ducklings on the foamy banks and a solitary Whooper Swan looking rather out of place below the castle ruins.

One of very few authentic French Art Deco pieces in a public British collection. This by Jacques Gruber — Art Deco stained glass window by Jacques Gruber held at The Bowes Museum

The pseudo chateau of The Bowes Museum, Barnard Castle — The Bowes Museum, Barnard Castle

The Butterbur, Petasites hybridus — Butterbur on the banks of The Tees

Yellow mallard/hybrid duckling on the Tees — Yellow hybrid Mallard duckling on the Tees

Solitary Whooper Swan, Cygnus cygnus, on the Tees at Barnard Castle — Solitary Whooper Swan, Barnard Castle

Common Redpoll photographed through a kitchen window — Redpoll photographed through the kitchen window

Teesdale chicken — Gallus gallus domesticus

ChemistGPT

TL:DR – Recent experience with asking ChatGPT chemistry questions reveals the limitations of this language model, despite the claims and hype of an ignorant US politician speaking in March 2023.

There was lots of talk of ChatGPT apparently deciding to teach itself organic chemistry…now…ChatGPT is language model, it’s not really an artificial intelligence (AI) in the strict sense, all it really is is a sophisticated autocomplete that analyses your prompts and weighs up the most likely combination of words with which to reply. I wondered whether the only chemistry in all that hype was the chemistry between olfactory receptor and odourant molecules in bovine ordure. So, I set it a challenge, giving it the following prompt:

You are to act as an expert organic chemist. You need to devise a reaction scheme using retrosynthetic analysis for the drug molecule Taxol.

This is how it responded:

Taxol is a complex natural product that has antitumor activity and is used in the treatment of various cancers. The molecule contains a complex array of functional groups, including multiple stereocenters, which make its synthesis a significant challenge.

Here is a possible retrosynthetic analysis of taxol:

Step 1: The first step involves disconnection of the C13 side chain from the taxane core. This can be achieved using a standard acid-catalyzed esterification reaction between a carboxylic acid and an alcohol. This disconnection leads to the formation of the intermediate 10-deacetylbaccatin III.

Step 2: The second step involves disconnection of the A and B rings from the taxane core. This can be achieved using a retro-Diels-Alder reaction. The retro-Diels-Alder reaction involves breaking the six-membered ring formed in the Diels-Alder reaction between the A and B rings. This leads to the formation of the intermediate 9-dihydro-13-acetylbaccatin III.

Step 3: The third step involves disconnection of the C ring from the taxane core. This can be achieved using a ring-opening reaction. The ring-opening reaction involves breaking the bond between C9 and C10, leading to the formation of the intermediate 7,8-didehydro-10-deacetylbaccatin III.

Step 4: The fourth step involves disconnection of the D ring from the taxane core. This can be achieved using a ring-closing reaction. The ring-closing reaction involves forming a new bond between C1 and C14, leading to the formation of the intermediate 5-deoxy-4-O-methyltaxol.

Step 5: The final step involves disconnection of the C2 side chain from the taxane core. This can be achieved using a standard nucleophilic substitution reaction. This leads to the formation of the final product, taxol.

Overall, the retrosynthetic analysis of taxol involves a series of complex reactions, including esterification, retro-Diels-Alder reaction, ring-opening reaction, ring-closing reaction, and nucleophilic substitution reaction. These reactions can be challenging to carry out in practice, and developing efficient and practical synthetic routes to taxol remains an active area of research in organic chemistry.

Now, it’s a long time since I did any retrosynthetic analyses for organic chemistry and I was never any good at them when I was meant to be. So, I’ll put this to social media and see what some proper chemists think.

First comment in was from ChemJobber, who said “the first disconnection to start with 10-deacetylbaccatin is reasonable”

I wrote about the first total synthesis of Taxol back in the 90s, and recall that the overall reaction scheme had well over 20 steps…

Nessa Carson dissected it and found the ChatGPT scheme rather lacking – here. She also pointed out that in Step 5 it completes the retrosynthesis of Taxol by disconnecting it all the way back to… Taxol (lol)

Red-green Carpet

TL:DR – It’s spring and at least one hibernating moth, the Red-green Carpet, has arrived in our garden so far this year

Lit up with my Heath trap again last night and despite the rain, but perhaps because it didn’t drop below 9 Celsius there was a small clutch of moths to ID and record this morning. Specifically, 2x Clouded Drab (new for the year), Common Quaker, Early Grey, 2x Hebrew Character, and a Red-green Carpet, also NFY.

Red-green Carpet (Chloroclysta siterata) — Red-green Carpet, Chloroclysta siterata

The Red-green Carpet is usually on the wing in the Autumn, the males die, but the females can hibernate to lay eggs in the spring. To my eye, they generally look green with some spots and patches at this time of year, but the autumnal ones are more obviously green and red…ish.

The “carpet” part of its name refers not to the notion that its larvae eat carpets, rather than in the 1700s when species were first being classified and named, the early entomologists and indeed the Lepidopterists perceived the beauty of these creatures and equated their colourful patterns and markings as being like the luxury items carpets were at the time.

The Red-green Carpet moth is a beautiful and fascinating species of moth that belongs to the family Geometridae, which includes many other species commonly known as “carpet moths”. This moth is found throughout Europe, including the United Kingdom, where it is one of the most common species of carpet moth.

Like all Lepidoptera, the species undergoes a complete metamorphosis, with four distinct life stages: egg, larva, pupa, and adult. The eggs are laid on the leaves of the host plant, which can include a wide variety of deciduous trees, most commonly oak and rowan. The larvae, which are often called “inchworms” (US usage) because of their distinctive way of moving, measuring the earth…hence geometers undergo several moults before pupating. The pupa is a non-feeding, transformative stage in which the larva transforms into the adult moth. Finally, the adult emerges from the pupa and begins the cycle anew by finding a mate and the females laying eggs.

The northern lights aren’t in my eyes – Aurora borealis

TL:DR – The Northern Lights, Aurora Borealis and their antipodean equivalent, the Southern Lights, Aurora Australis, are a visible phenomenon seen in the polar skies as particles from the solar wind interact with particles in the earth’s upper atmosphere.

Lots of lucky locals, by which I mean people a bit further north in Norfolk saw the northern lights, the Aurora Borealis, in Norfolk and elsewhere. There is a slight possibility of seeing them in Cambridgeshire although finding somewhere with little light pollution around here is a tough call, but more to the point it’s been cloudy and wet when other places have had their lightshow these last couple of nights. In recent years they have been observed from Devon and Cornwall.

Aurora borealis at Vestrahorn, Southern Iceland by German photographer Simaron — Aurora borealis at Vestrahorn, Southern Iceland (Credit: Simaron)

So, what are the northern lights?

The northern lights are a natural phenomenon generally occurring close to the poles, in the high or low latitude regions of the Arctic and Antarctic. The phenomenon is caused by the interaction between charged particles from the Sun and the Earth’s protective magnetic field.

The Sun constantly emits a stream of charged particles, called the solar wind, which travels through space and interacts with the Earth’s magnetic field. When these charged particles collide with the oxygen and nitrogen in the Earth’s atmosphere, they gain energy, they are excited, when the excitement passes, the particles release energy in the form of light. This produces the glowing, coloerful display that we see as the Northern Lights, or the Southern Lights, the Aurora Australis.

The colours of the Northern Lights depend on the type of gas particles that are colliding with the charged particles and the altitude at which the excitement occurs. Oxygen at higher altitude will glow red and at lower altitude will grown green. Nitrogen produces blue and purple hues.

Usually, seeing the Aurora Borealis involves heading to the colder regions Iceland, Norway, Sweden, Finland, or Canada during the winter months and hoping for a strong solar wind, caused by lots of activity on the surface of the sun. Occasionally, activity and conditions are just right for people to see them farther from the poles as has happened in recent days.

Aurora comes from the name of the Roman goddess of the dawn. It’s related to the name of the Indo-European goddess of the dawn and ultimately the root is from ancient Greek “to shine” with particular reference to the dawn sky.

Borealis comes from the Latin Boreas meaning the “north wind” and from the Greek Boreas, also the name of the god of the north wind.

Australis derives from the Latin word auster meaning “south wind” and hence relates to the southern part of the world.

I haven’t ever managed to see nor photograph the Northern nor the Southern Lights hence the allusion to the lyrics of a 1978 song by progressive rock band Renaissance. The singer with the band, Annie Haslam, was renowned for her operatic training and her five-octave vocal range. I recently updated an article you might like on how to sing.

The photo above was taken by Simaron at Vestrahorn, Southern Iceland in September 2022.

Early Oak Piercer just for FUN

TL:DR – Pheromones are a useful tool for discovering what day-flying moths are in your neighbourhood.

As regular readers know, I do a bit of mothing…I have had a FUN lure, a pheromone lure aimed at attracting Grapholita funebrana. It’s been sighted in the garden for a couple of weeks with nothing appearing until 21st March 2023 at which point I was lucky enough to catch a couple of micro moths, Pammene giganteana (known to some unofficially as the Early Oak Piercer) not the target species but nice to see, nevertheless.

The micro moth Pammene giganteana (Early Oak Piercer) — The micro moth *Pammene giganteana* (Early Oak Piercer)

The larvae of the Early Oak Piercer feed inside oak-apple galls, which are themselves formed by parasitic wasps.

According to Anglian Lepidopterist Supplies from whom I bought this and other pheromone lures, the FUN lure has a long list of non-target species that might be drawn to it at different times of year, there may be others yet to be reported:

Glyphipterix fuscoviridella , Phyllonorycter quercifoliella, Grapholita janthinana , Cnephasia stephensiana , Ochsenheimeria vacculella, Epiblema costipunctana, Grapholita tenebrosana, Pseudargyrotoza conwagana, Dichrorampha aeratana, Acentria ephemerella, Argyresthia curvella, Endothenia gentianaeana, Hadena confusa (Marbled Coronet), Lymantria dispar (Gypsy Moth), Agrotis segetum (Turnip Moth), Tinea semifulvella, Pammene argyrana, Pammene aurana, Pammene giganteana, Pammene albuginana, Pammene obscurana, Pammene suspectana, Pammene fasciana, Amblyptilia punctidactyla

Pheromone moth trap — A simple pheromone moth trap. The lure is held in a receptacle above an funnelled opening, a moth attracted to the lure will commonly fall into the funnel and be unable to escape, ready for identification, logging, and release back into the wild unharmed.

Alpine Swifts in the UK and Ireland

TL:DR – The bird report pages noted what seemed to be quite an unusual number of Alpine Swifts across the UK during March 2023.

Back in 2019, we took a trip to Greece, the first in many years, we saw lots of wildlife, including Alpine Swift, Tachymarptis melba, careening way above our heads in Athens.

The alpine swift is a medium-sized bird with a wingspan of around 540-600 mm. It has a dark-brown body with a slightly paler throat and underbelly. In flight, it is easily identified by its long, narrow wings and its distinctive white belly patch, which contrasts sharply with the dark body. It is a skilled and agile flier, capable of catching insects on the wing with great precision. Indeed, it rarely touches down, spending almost its whole life, once fledged on the wing, except perhaps when incubating eggs in their nests. It will only very rarely land on the ground.

Alpine Swifts flying against a blue sky above central Athens, 2019 — Alpine Swifts

During the breeding season, which typically runs from May to August, Alpine Swifts build their nests in crevices and holes in the rocky cliffs and mountainsides of southern Europe to the Himalaya. They use their saliva to glue together small twigs and feathers, forming a shallow, cup-shaped nest. The female lays a clutch of 2-3 eggs, both parents take turns to incubate the eggs for around three weeks.

After the breeding season, the Alpine Swift migrates south to spend the winter in sub-Saharan Africa. They are known for their long-distance flights and are capable of covering up to 1000 km in a 24-hour period. They will return to the same nest sites year after year.

As I was writing this, yet another “ping” came in on my rare bird sightings app to alert me to another Alpine Swift having been spotted in the UK. It’s March 2023. Quite early for the Common Swift, which we always see in the UK each summer. Alpine Swifts though? Very unusual. They have been sighted in numerous places in the UK and Ireland this spring.

Sightings have been reported since mid-March this year in the following counties: Antrim, Armagh, Cheshire, Cork, Cornwall, Devon, Dorset, Down, Dublin, Dumfries & Galloway, East Sussex, Essex, Forth, Glamorgan, Gloucestershire, Isle of Man, Isle of Wight, Kent, Leitrim, Lincolnshire, London, Lothian, Mayo, Norfolk, Northumberland, Pembrokeshire, Somerset, Waterford, Wicklow, Yorkshire.

The early reports were marked as “notable” rather than “mega”. In 2022, there was just one noted in March, although later in the year a few others were sighted. Similarly for previous years. So, while I admit, I’d not known about their habits this far north and west of southern Europe, it does seem that a large number have appeared in British and Irish skies very early in the spring this year.

One cannot make generalisations about animal behaviour based on data from a single year, of course. However, the appearance of Alpine Swifts in numbers, this early in the year, suggests something may have changed for them this period. It could simply be changes in weather patterns and wind currents, whether or not those are due to climate change is a different matter. It is known that Alpine Swifts to range quite widely during their migration so maybe this is not an aberration at all and just a reversion to a pattern they followed some time in the distant past.

Stephen Moss discussed the recent irruption of Alpine Swifts in The Guardian some time after my original post and alluded to the fact that because of climate change, this species may well begin breeding in the UK at some point in the next few years, if it hasn’t already done so.

The flour beetle’s water butt

TL:DR – A new study has demonstrated the ability of red flour beetles (Tribolium castaneum) to recycle water in their rectums. This feat allows the beetles to survive in some of the driest environments on Earth.

The research team of Kenneth Halberg and colleagues built a transcriptomic atlas of the beetles’ life stages, which allowed them to systematically compare gene expression across tissues and stages. By searching the atlas for genes with enriched expression in the rectum, the researchers were able to identify a specific gene associated with this phenomenon, Nha1.

Electrophysiological experiments confirmed that Nha1 plays a crucial role in ion transport in rectal cells and water uptake by the rectum. In fact, silencing the expression of Nha1 led to water loss, emphasizing the importance of this gene in the beetle’s survival.

Red flour beetles have a large surface-to-volume ratio that makes them particularly vulnerable to environmental water loss. However, the beetles’ rectal complex enables them to recycle water from their faeces and even harvest water from moist air. This remarkable ability is key to the beetle’s survival in arid settings.

The findings of this study shed light on the evolution of water recycling and conservation mechanisms in insects. Furthermore, the transcriptomic atlas of red flour beetles, known as BeetleAtlas, can be used to address a range of unresolved questions on Tribolium biology. Overall, this research highlights an intriguing adaptation that allows insects to thrive in challenging environments.

“NHA1 is a cation/proton antiporter essential for the water-conserving functions of the rectal complex in Tribolium castaneum,” by Muhammad T. Naseem et al. Proc Natl Acad Sci

British mothers in the early spring

TL:DR – Mothing is a simple, but educational and fascinating hobby. The data it accumulates can be useful scientifically.

I have lit up with a couple of different traps through the winter, but with generally disappointing results. There are not a lot of trees in our neighbourhood, which I suspect is the reason we don’t get a huge number of moths. That said, very few moth-ers see lots of moths in the gardens during the winter months.

15Watt Heath type, portable moth trap in place and ready for lighting up time

I put out my “spare” Heath trap last night. It is basically an ultraviolet fluorescent tube supported above funnel on top of a plastic box. The UV tube attracts nocturnal moths. They flap about a bit around the tube, perhaps bash into the vertical plastic vanes, and drop into the box. The box contains a load of cardboard egg cartons and the moths will generally secrete themselves among the hollows once they’re bored with chasing the light.

Come the morning, the diligent moth-er will be up at dawn to identify and count the moths trapped overnight, record the data ready to send off to the county moth recorder for scientific analysis along with records from others. The moths themselves are released off-site into bushes and undergrowth, preferably near dusk, and safely carry on with their lives.

Quick phone macro shot of the Hebrew Character moth and in the background an Early Grey

The haul from the moth trap, if you could call it a haul, was the biggest and most diverse of the year so far. Four moths of three different species. A micro moth known as Diurnea fagella, which arrived about three weeks earlier than this in 2022, a second Hebrew Character, and the first Early Grey, two of them. At the height of summer, I expect to see a couple of hundred moths of 60 differents species on a lighting up night. But, it was just 7 Celsius overnight, that and other factors at play can keep numbers down until well into the spring it seems.

Now, you are perhaps wondering whether this is ethical or even worse cruel and there are definitely arguments for and against. But, what is definitely known is that we need to monitor the natural world to know anything about it with the aim of helping protect it, conserve, and restore. The moths are just one indicator of whether a location is environmentally healthy. They are often expert pollinators. They and their larvae also provide food for birds, frogs, and various other animals. They are themselves beautiful and fascinating creatures and deserve their place in the world just as any other living creature. I wrote on this issue about a year after I started mothing.

Oh, and if you were worrying about your woolen carpet and your three-piece suit, there are actually something like 1500 different species of moth in the UK, and only one or two species have larvae that eat textiles.

Incidentally, it was some of the names that first drew me to mothing. The one I mentioned above, Hebrew Character is fascinating and closely related etymologically to the Setaceous Hebrew Character.

The orgasmic heart beat

TL:DR – Sex can double or treble a person’s heart rate, but this is a normal physiological response provided the rate goes back to normal within a few minutes.

Love might make your heart skip a beat, but love-making definitely gets it pumping. Indeed, sexual activity will inevitably raise your bpm, beats per minute, especially as a person approaches orgasm. A friend with a fake FitBit was curious about the trajectory of heart rate during sex and wore his monitor (around his wrist) in the bedroom one night. As he and his missus slid between the sheets, he set it to “other” workout and then synced the output from the monitor to his phone…the morning after.

Photo via Pexels user https://www.pexels.com/@olly/

My friend was astonished to see that his heart ultimately raced up to 156 bpm. That is within what the fitness app refers to as the anaerobic region. He was relieved to see that it fell to a near-normal resting rate within a couple of minutes afterwards though. But, that peak had him worried, especially at his age. So rather sheepishly he asked me, as his go-to-science friend, to check out the numbers.

I assumed he knew that during sexual activity, the body undergoes various physiological changes, including an increase in heart rate. It is normal for the heart rate to increase during sexual arousal and activity, and this increase is generally not a cause for concern in healthy individuals.

Heart rate recorded by a fake fitbit during sex

But, it was the orgasm bpm he was worried out. During orgasm, the heart rate typically reaches its peak, and in some people, it can exceed 180 bpm. However, this increase in heart rate is usually brief and does not pose a significant risk to health. 156 bpm during orgasm is within the normal range for sexual activity. And, the fact that his heart rate quickly returned to around 80 bpm within a couple of minutes is a healthy response and indicates that his cardiovascular system is functioning properly.

Certainly, there would be something to worry about if it had gone very high and stayed at that level for a prolonged period. Moreover, chest tightness and pain, stabbing or shooting pains in the left arm or pain in the neck or jaw would indicate a need to seek medical attention either urgently or sooner, rather than later.

As an aside, my friend finds it amusing that he does a lot more “steps” when he is alone than when he’s with his missus…now…I know some readers are going to think “friend…? Yeah, right!” But, it’s true.

Sexual activity, including arousal and orgasm, causes the release of hormones such as adrenaline, noradrenaline, and dopamine, which can increase heart rate. Additionally, physical activity during sex, such as thrusting or movement, also contributes to the rise in heart rate. The body also experiences increased blood flow during sexual activity, which places an additional demand on the heart to pump blood. All of these factors work together to increase heart rate during sexual activity.

Increased blood flow is necessary during sexual activity to deliver oxygen and nutrients to the muscles and tissues involved in the sexual response. This includes the genitalia, which require increased blood flow to become engorged and maintain an erection in men and to lubricate and swell in women. The increased blood flow also contributes to the overall sensations of sexual arousal and pleasure. Increased blood flow to the genitals facilitates sexual function and satisfaction.

During orgasm, the male heartbeat can exhibit variations. While some individuals may experience a sensation akin to their heart skipping a beat, it’s not a universal occurrence. The physiological response during orgasm involves a complex interplay of various bodily systems, including the cardiovascular system.

Typically, during sexual arousal and orgasm, there’s an increase in heart rate and blood pressure as a result of heightened arousal and the release of adrenaline. This can lead to palpitations or a perceived skipping sensation in some individuals. Additionally, the release of neurotransmitters such as dopamine and oxytocin during orgasm can also impact heart rate and rhythm.

However, it’s important to note that variations in heartbeat during orgasm can differ greatly from person to person, and not everyone may perceive or experience it in the same way. If someone experiences significant irregularities in heartbeat or has concerns about their cardiovascular health during sexual activity, it’s advisable to consult with a healthcare professional for further evaluation and guidance.